Johne's Disease Acid Fast v. FISH Images
Fibrosis~ Kirk Harris uses sequencing to analyze bacterial community composition in lung sputum and bronchial lavages of
CF patients. This is useful because frequently the exacerbation-causing organisms are not brought into culture in the hospital lab. Potentially, this work could be used to drive treatment of CF patients based on the bacterial pathogens identified through sequencing.
An ongoing study of the microbial community composition
in the intestines of Crohn's Disease cattle. This is
a gastro-intestinal disease that may be caused by bacteria
and is becoming more prevalent. Previous studies in
the lab have studied inflammatory bowel disease (IBD)
and chrone's disease in humans.
Methicillin-resistant Staphylococcus aureus (MRSA)
represents an increasing risk especially in hospital
increasingly common skin condition in children, causes
inflammation, lesions, and itchiness. Often associated
with microbial infection.
inflamation of the prostate affects 50% of all men at
some point in their life. Prostatitis maybe bacterial
or non-bacterial by hospital culture results.
Past Projects and Publications
Frank, D.N., L.M. Feazel, M.T. Bessesen, C.S. Price, E.N. Janoff and N.R. Pace. (2010) The human nasal microbiota and Staphylococcus aureus carriage. PLoS ONE 5:e10598 (15 pp.).
Frank, D.N., S.S. Wilson, A.L. St. Amand, and N.R. Pace. (2009) Culture-independent microbiological analysis of Foley urinary catheter biofilms. PLoS ONE 4:e7811 (7 pp.).
Frank, DN, Wysocki, A, Specht-Glick, DD, Rooney, A, Feldman, RA, St Amand, AL, Pace, NR, and Trent, JD (2009) Microbial diversity in chronic open wounds.
Wound Repair Regen 17(2):163-72.
Frank, DN (2008) XplorSeq: A software environment for integrated management and phylogenetic analysis of metagenomic sequence data. BMC Bioinformatics 9: 420.
Frank, DN and Pace, NR (2008) Gastrointestinal microbiology enters the metagenomics era. Curr Opin Gastroenterol, 24(1):4-10.
Peterson, DA, Frank, DN, Pace, NR, and Gordon, JI (2008) Metagenomic approaches for defining the pathogenesis of inflammatory bowel diseases.
Cell Host Microbe 3(6):417-27.
Harris JK, De Groote MA, Sagel SD, Zemanick ET, Kapsner
R, Penvari C, Kaess H, Deterding RR, Accurso FJ, Pace
NR.(2007) Molecular identification of bacteria in bronchoalveolar
lavage fluid from children with cystic fibrosis.Proc
Natl Acad Sci 104(51):20529-33.
DN, St Amand AL, Feldman RA, Boedeker EC, Harpaz N,
Pace NR. (2007) Molecular-phylogenetic characterization
of microbial community imbalances in human inflammatory
bowel diseases. Proc Natl Acad Sci. 104(34):13780-5
Amand AL, Frank DN, De Groote MA, Basaraba RJ, Orme
IM, Pace NR. (2005) Use of specific rRNA oligonucleotide
probes for microscopic detection of Mycobacterium tuberculosis
in culture and tissue specimens. J Clin Microbiol. 2005
Gardner EM, Burman WJ, DeGroote MA, Hildred G, Pace
NR. (2005) Conventional and molecular epidemiology of
macrolide resistance among new Mycobacterium avium complex
isolates recovered from HIV-infected patients. Clin
Infect Dis. 41(7):1041-4.
St Amand AL, Frank DN, De Groote MA, Pace NR.(2005)
Use of specific rRNA oligonucleotide probes for microscopic
detection of Mycobacterium avium complex organisms in
tissue. J Clin Microbiol. 43(4):1505-14
Angenent LT, Kelley ST, St Amand A, Pace NR, Hernandez
MT. (2005) Molecular identification of potential pathogens
in water and air of a hospital therapy pool. Proc Natl
Acad Sci U S A. 102(13):4860-5.
De Groote MA, Frank DN, Dowell E, Glode MP, Pace NR.
(2005) Lactobacillus rhamnosus GG bacteremia associated
with probiotic use in a child with short gut syndrome.
Pediatr Infect Dis J. 24(3):278-80.
Frank DN, Spiegelman GB, Davis W, Wagner E, Lyons E,
Pace NR. (2003) Culture-independent molecular analysis
of microbial constituents of the healthy human outer
ear. J Clin Microbiol. 41(1):295-303.