The Pace Lab

University of Colorado-Boulder

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RNA Projects

Continued Analysis of Bacterial RNase P Structure~ The fine structure of bacterial Ribonuclease P is still being resolved by comparing structures from different bacteria. Kinetics, mutational analyses, covariation, and X-RAY crystallography, are techniques commonly utilized by the P researchers: Alexei, Lika, Donnie, Katrina and Sina.

EcoliP

Eucaryal RNase P~ Steve Marquez studies the RNase P molecule of eucaryotic organisms. In eucaryotes there are many more affiliated proteins needed for the catalytic function of RNase P.

Recent Publications

Kazantsev, AV, Krivenko, AA, and Pace, NR (2008) Mapping metal-binding sites in the catalytic domain of bacterial RNase P RNA.
RNA 15: 266-276.

Marquez SM, Chen JL, Evans D, Pace NR. (2006) Structure and function of eukaryotic Ribonuclease P RNA. Mol Cell. 24(3):445-56.

Dalby AB, Frank DN, St Amand AL, Bendele AM, Pace NR. (2006) Culture-independent analysis of indomethacin-induced alterations in the rat gastrointestinal microbiota. Appl Environ Microbiol. 72(10):6707-15.


Kazantsev AV, Pace NR.(2006) Bacterial RNase P: a new view of an ancient enzyme.
Nat Rev Microbiol. 4(10):729-40. Review.

Evans D, Marquez SM, Pace NR. (2006) RNase P: interface of the RNA and protein worlds. Trends Biochem Sci. 31(6):333-41.

Marquez SM, Chen J, Evans D, Pace NR. (2006) Structure and function of eukaryotic ribonuclease P RNA. Mol. Cell. 24:445-56.

Kazantsev AV, Pace NR. (2006) Bacterial RNase P: a new view of an ancient enzyme. Nature Rev. Microbiol. 4:729-40.

Buck AH, Kazantsev AV, Dalby AB, Pace NR. (2005) Structural perspective on the activation of RNAse P RNA by protein. Nat Struct Mol Biol. 12(11):958-64.

Buck AH, Dalby AB, Poole AW, Kazantsev AV, Pace NR. (2005) Protein activation of a ribozyme: the role of bacterial RNase P protein. EMBO J. 24(19):3360-8.

Kazantsev AV, Krivenko AA, Harrington DJ, Holbrook SR, Adams PD, Pace NR. (2005) Crystal structure of a bacterial ribonuclease P RNA. Proc Natl Acad Sci U S A. 102(38):13392-7.


Marquez SM, Harris JK, Kelley ST, Brown JW, Dawson SC, Roberts EC, Pace NR. (2005) Structural implications of novel diversity in eucaryal RNase P RNA. RNA. 11(5):739-51

Kazantsev AV, Krivenko AA, Harrington DJ, Carter RJ, Holbrook SR, Adams PD, Pace NR. (2003) High-resolution structure of RNase P protein from Thermotoga maritima. Proc Natl Acad Sci U S A. 100(13):7497-502


Krivenko AA, Kazantsev AV, Adamidi C, Harrington DJ, Pace NR. (2002) Expression, purification, crystallization and preliminary diffraction analysis of RNase P protein from Thermotoga maritima. Acta Crystallogr D Biol Crystallogr. 58(Pt 7):1234-6.

Li X, Frank DN, Zengel JM. (2002) Phylogenetic analysis of the strucutre of RNase MRP RNA in yeasts. RNA. 8:741-52

Lab News

August 2006

Donnie Evans and Steve Marquez publish a review on the emerging view of coevolution betwen RNaseP and its associated proteins  

 

A Sample of Sequence Alignment

 

alignment