RNA Projects

Continued Analysis of Bacterial RNase P Structure~ The fine structure of bacterial Ribonuclease P is still being resolved by comparing structures from different bacteria. Kinetics, mutational analyses, covariation, and X-RAY crystallography, are techniques commonly utilized by the P researchers: Alexei, Lika, Donnie, Katrina and Sina.

Eucaryal RNase P~ Steve Marquez studies the RNase P molecule of eucaryotic organisms. In eucaryotes there are many more affiliated proteins needed for the catalytic function of RNase P.

Recent Publications

Evans D, Marquez SM, Pace NR. (2006) RNase P: interface of the RNA and protein worlds. Trends Biochem Sci. 31(6):333-41.

Marquez SM, Chen J, Evans D, Pace NR. (2006) Structure and function of eukaryotic ribonuclease P RNA. Mol. Cell. 24:445-56.

Kazantsev AV, Pace NR. (2006) Bacterial RNase P: a new view of an ancient enzyme. Nature Rev. Microbiol. 4:729-40.

Buck AH, Kazantsev AV, Dalby AB, Pace NR. (2005) Structural perspective on the activation of RNAse P RNA by protein. Nat Struct Mol Biol. 12(11):958-64.

Buck AH, Dalby AB, Poole AW, Kazantsev AV, Pace NR. (2005) Protein activation of a ribozyme: the role of bacterial RNase P protein. EMBO J. 24(19):3360-8.

Kazantsev AV, Krivenko AA, Harrington DJ, Holbrook SR, Adams PD, Pace NR. (2005) Crystal structure of a bacterial ribonuclease P RNA. Proc Natl Acad Sci U S A. 102(38):13392-7.

Marquez SM, Harris JK, Kelley ST, Brown JW, Dawson SC, Roberts EC, Pace NR. (2005) Structural implications of novel diversity in eucaryal RNase P RNA. RNA. 11(5):739-51

Kazantsev AV, Krivenko AA, Harrington DJ, Carter RJ, Holbrook SR, Adams PD, Pace NR. (2003) High-resolution structure of RNase P protein from Thermotoga maritima. Proc Natl Acad Sci U S A. 100(13):7497-502

Krivenko AA, Kazantsev AV, Adamidi C, Harrington DJ, Pace NR. (2002) Expression, purification, crystallization and preliminary diffraction analysis of RNase P protein from Thermotoga maritima. Acta Crystallogr D Biol Crystallogr. 58(Pt 7):1234-6.

Li X, Frank DN, Zengel JM. (2002) Phylogenetic analysis of the strucutre of RNase MRP RNA in yeasts. RNA. 8:741-52

August 2006

Donnie Evans and Steve Marquez publish a review on the emerging view of coevolution betwen RNaseP and its associated proteins